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vectashield plus antifade mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield plus antifade mounting medium with dapi
    Vectashield Plus Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21861 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectashield+plus+antifade+mounting+medium/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc12856636-82-21-28
    Average 98 stars, based on 21861 article reviews
    vectashield plus antifade mounting medium with dapi - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Novel mechanisms of chemosensory adaptation to the cave environment
    Article Snippet: .. Following IHC or HCR-FISH, several single lamellae per olfactory epithelial rosette were dissected, and placed on within a circle drawn with PAP pen (ImmEdge Hydrophobic Barrier Pen: Vector Laboratories, Newark, CA, USA) at a slide glass (Corning Incorporated, Corning, NY, USA) using VECTASHIELD PLUS Antifade Mounting Medium (Vector Laboratories, Inc., Newark, CA, USA) and sealed with a cover glass (Fisher Scientific, Pittsburgh, PA, USA). .. For cryosections, labeled olfactory epithelial rosettes were immersed in a series of sucrose gradients (VWR International) in 1× PBS: 5% Sucrose in PBS for 3 hours at 4°C, 10% sucrose in PBS for 4 hours at 4°C, and 20% sucrose in PBS overnight at 4°C.

    Olfactory:

    Article Title: Novel mechanisms of chemosensory adaptation to the cave environment
    Article Snippet: .. Following IHC or HCR-FISH, several single lamellae per olfactory epithelial rosette were dissected, and placed on within a circle drawn with PAP pen (ImmEdge Hydrophobic Barrier Pen: Vector Laboratories, Newark, CA, USA) at a slide glass (Corning Incorporated, Corning, NY, USA) using VECTASHIELD PLUS Antifade Mounting Medium (Vector Laboratories, Inc., Newark, CA, USA) and sealed with a cover glass (Fisher Scientific, Pittsburgh, PA, USA). .. For cryosections, labeled olfactory epithelial rosettes were immersed in a series of sucrose gradients (VWR International) in 1× PBS: 5% Sucrose in PBS for 3 hours at 4°C, 10% sucrose in PBS for 4 hours at 4°C, and 20% sucrose in PBS overnight at 4°C.

    Staining:

    Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
    Article Snippet: After rinsing in PBS, sections were incubated with Alexa Fluor 594 secondary antibody (Anti-goat, raised in donkey; 1:500; Cat#A11058; Invitrogen, USA) for 4 hours at room temperature. .. Finally, sections were rinsed thrice in PBS, mounted on gelatin-coated slides, air-dried, and coverslipped with Vectashield® Plus Antifade mounting medium containing DAPI nuclear stain (H-2000, Vector Laboratories, USA). .. Somatostatin-positive neurons at different stages of development were quantified using the optical fractionator method (Stereoinvestigator software; Microbrightfield, Williston, VT) attached to an AR TI CL E IN P RE SS ARTICLE IN PRESS Olympus microscope (BX51).



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    96
    Vector Laboratories vectashield plus antifade mount medium with dapi
    (a) (b) Representative images of immunofluorescently stained carotid cross sections from sham-operated and ligated controls, moderate EtOH, and Binge EtOH experimental groups (males). Blue = <t>Dapi</t> nuclear stain, red = Tm-Cdh5+, and white=αSMA+. (c) Cells co-expressing Cdh5 and αSMA (i.e., myo-endothelial cells indicative of EndMT) were quantified using QuPath bioimage analysis software in carotid cross sections post-ligation from controls (grey bars), moderate EtOH (green bars), and binge EtOH (red bars) experimental groups. Bar graphs show cumulative data expressed as % Myoendothelial cells /full cross section, % Myoendothelial cells /neointima, or Number of myoendothelial cells/full cross section. Data are mean±SEM, n=25-30 sections from 5-6 mice). *p<0.05, ** P<0.001, **** p<0.0001.
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    Image Search Results


    (a) (b) Representative images of immunofluorescently stained carotid cross sections from sham-operated and ligated controls, moderate EtOH, and Binge EtOH experimental groups (males). Blue = Dapi nuclear stain, red = Tm-Cdh5+, and white=αSMA+. (c) Cells co-expressing Cdh5 and αSMA (i.e., myo-endothelial cells indicative of EndMT) were quantified using QuPath bioimage analysis software in carotid cross sections post-ligation from controls (grey bars), moderate EtOH (green bars), and binge EtOH (red bars) experimental groups. Bar graphs show cumulative data expressed as % Myoendothelial cells /full cross section, % Myoendothelial cells /neointima, or Number of myoendothelial cells/full cross section. Data are mean±SEM, n=25-30 sections from 5-6 mice). *p<0.05, ** P<0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal Transition

    doi: 10.64898/2026.04.14.718463

    Figure Lengend Snippet: (a) (b) Representative images of immunofluorescently stained carotid cross sections from sham-operated and ligated controls, moderate EtOH, and Binge EtOH experimental groups (males). Blue = Dapi nuclear stain, red = Tm-Cdh5+, and white=αSMA+. (c) Cells co-expressing Cdh5 and αSMA (i.e., myo-endothelial cells indicative of EndMT) were quantified using QuPath bioimage analysis software in carotid cross sections post-ligation from controls (grey bars), moderate EtOH (green bars), and binge EtOH (red bars) experimental groups. Bar graphs show cumulative data expressed as % Myoendothelial cells /full cross section, % Myoendothelial cells /neointima, or Number of myoendothelial cells/full cross section. Data are mean±SEM, n=25-30 sections from 5-6 mice). *p<0.05, ** P<0.001, **** p<0.0001.

    Article Snippet: Cells were incubated with primary antibody in 1/10 th gelatin in PBS for 2-3 h. Following 3X washes, cells were incubated with flourescently-labelled secondary antibody for 1-2 h. Following 3x washes cells were mounted on slides using Vectashield Plus Antifade mount medium with DAPI (Cat. # H20002, Vector Laboratories) and scanned with confocal microscope (Nikon A1R HD, Pikachu).

    Techniques: Staining, Expressing, Software, Ligation